B-CAG-hB7-H3 MC38

Basic Information

Strain Name
MC38 Cd276tm1(hCD276)/Bcgen
Stock Number
310704
Common Name
B-CAG-hB7-H3 MC38
Disease
Colon carcinoma
Species
Mus musculus, mouse
Growth factors
Adherent
Culture medium
DMEM+10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-hB7-H3 MC38.The exons 3-4 of mouse B7-H3 gene that encode the extracellular domain were replaced by human B7-H3 CDS region in B-CAG-hB7-H3 MC38.

Details

Protein expression analysis

The B7-H3 expression analysis in B-CAG-hB7-H3 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-CAG-hB7-H3 MC38 cells, and analyzed by flow cytometry with species-specific anti-B7-H3 antibody. Human B7-H3 was exclusively detectable in B-CAG-hB7-H3 MC38 cells but not wild-type MC38 cells.

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-hB7-H3 MC38 cells. B-CAG-hB7-H3 MC38 cells and wild-type MC38 cells (5×105) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-hB7-H3 MC38 were able to establish tumor in vivo and can be used for efficacy study.

Protein expression analysis in tumor cells

B-CAG-hB7-H3 MC38 tumor cells were transplanted into C57BL/6N mice(n=5), and on 31 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells. As Shown,human B7-H3 was highly expressed on the surface of tumor cells. B-CAG-hB7-H3 MC38 can be used for in vivo efficacy validation.

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