B-hBCMA MC38

Basic Information

Description

 The MC38 cell line is derived from C57BL/6 murine colon adenocarcinoma cells. The cell line is a commonly used murine model for colorectal carcinoma.

Targeting strategy

The exogenous promoter and human BCMA coding sequence were inserted into the mouse ROSA26 locus of wild MC38 cell lines.

 

Protein expression analysis

BCMA expression analysis in B-hBCMA MC38 cells by flow cytometry. 

Single cell suspensions from wild-type MC38 and B-hBCMA MC38 cultures were stained with species-specific anti-BCMA antibody A (provide a client) and Belantamab analog (in house). Human BCMA was detected on the surface of B-hBCMA MC38 cells but not wild-type MC38 cells. The 2-B01 clone of B-hBCMA MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hBCMA MC38 cells. 

B-hBCMA MC38 cells (5×10⁵) and wild-type MC38 cells (5×10⁵) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Subcutaneous homograft tumor growth of B-hBCMA MC38 cells. 

B-hBCMA MC38 cells (5×10⁵, 5×10⁶) and wild-type MC38 cells (5×10⁵) were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Subcutaneous tumor growth of B-hBCMA MC38 cells. 

B-hBCMA MC38 cells (5×10⁶) and wild-type MC38 cells (5×10⁵) were subcutaneously implanted into B-hCD3E mice (female, 6-8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². Results indicate that B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Protein expression analysis of tumor cells

BCMA expression evaluated on B-hBCMA MC38 tumor cells by flow cytometry. 

B-hBCMA MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5), and on 36 days post inoculation, tumor cells were harvested and assessed for human BCMA expression by flow cytometry using anti-human BCMA antibody A (provide a client). As shown, human BCMA was expressed on the surface of tumor cells. Therefore, B-hBCMA MC38 cells can be used for in vivo efficacy studies of novel BCMA therapeutics.

Protein expression analysis of tumor tissue

BCMA expression evaluated on B-hBCMA MC38 tumor cells by flow cytometry. 

B-hBCMA MC38 cells were subcutaneously transplanted into B-hCD3E mice (n=6). Upon conclusion of the experiment, tumor cells were harvested and assessed for human BCMA expression by flow cytometry using anti-human BCMA antibody Belantamab analog (in house). As shown, human BCMA was highly expressed on the surface of tumor cells. Therefore, B-hBCMA MC38 cells can be used for in vivo efficacy studies evaluating novel BCMA therapeutics.