Gene targeting strategy for B-CAG-hBCMA MC38. The ROSA26 locus of the mouse was inserted into the human BCMA CDS region in B-CAG-hBCMA MC38.
Protein expression analysis
The BCMA expression analysis in B-CAG-hBCMA MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-CAG-hBCMA MC38 cells, and analyzed by flow cytometry with species-specific anti-BCMA antibody. Human CD47 was detectable in B-CAG-hBCMA MC38 cells but not wild-type MC38 cells.
Tumor Growth Curve & Body Weight Changes
Subcutaneous xenograft tumor growth of B-CAG-hBCMA MC38 cells. B-CAG-hBCMA MC38 cells and wild-type MC38 cells (5×105) were subcutaneously implanted into the C57BL/6 mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-hBCMA MC38 were able to establish tumor in vivo and can be used for efficacy study.
Tumor expression detection sample information
Protein expression analysis in tumor cells
B-CAG-hBCMA MC38 cells were transplanted into C57BL/6 mice(n=5), and on 35 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells. As Shown,human BCMA was highly expressed on the surface of tumor cells. B-CAG-hBCMA MC38 can be used for in vivo efficacy validation.