Cell protein expression analysis
B-CAG-hCLDN18.2 A549 Cell Line were analyzed by flow cytometry.
Result: B-CAG-hCLDN18.2 A549 expressed human CLDN18.2 protein.
Tumor growth curve
B-CAG-hCLDN18.2 A549 cells were subcutaneously implanted into the B-NDG mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.
Results: B-CAG-hCLDN18.2 A549 cells were able to establish tumor model steadily and can effectively track tumor volume.
Tumor expression detection sample information
Protein expression analysis in tumor cells
B-CAG-hCLDN18.2 A549 was transplanted into B-NDG mice(n=5), and on 45 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells.
Results: Human CLDN18.2 protein expression in B-CAG-hCLDN18.2 A549 cells was significantly rose compared with A549(WT).