B-CAG-hCLDN18.2 A549 Cell Line

Basic Information

Strain Name
A549 Cldn18.2tm1(hCLDN18.2)/Bcgen
Stock Number
310791
Common Name
B-CAG-hCLDN18.2 A549 Cell Line
Disease
Carcinoma
Species
Human
Growth factors
Adherent
Culture medium
McCoy’s 5A+10%FBS+1%L-Glutamine+1%NEAA+1%Sodium pyruvate+1%HEPES
Frozen
95% Complete medium+5% DMSO

Targeting Strategy

Details

Cell protein expression analysis

B-CAG-hCLDN18.2 A549 Cell Line were analyzed by flow cytometry.

Result: B-CAG-hCLDN18.2 A549 expressed human CLDN18.2 protein.

Tumor growth curve

B-CAG-hCLDN18.2 A549 cells were subcutaneously implanted into the B-NDG mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Results: B-CAG-hCLDN18.2 A549 cells were able to establish tumor model steadily and can effectively track tumor volume.

Tumor expression detection sample information

Protein expression analysis in tumor cells

B-CAG-hCLDN18.2 A549 was transplanted into B-NDG mice(n=5), and on 45 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells.

Results: Human CLDN18.2 protein expression in B-CAG-hCLDN18.2 A549 cells was significantly rose compared with A549(WT).

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