Phenotypic analysis of B-CAG-hCLDN18.2 MC38 Cell Line
B-CAG-hCLDN18.2 MC38 Cell Line were analyzed by flow cytometry. The hCLDN18.2 protein was detected in B-CAG-hCLDN18.2 MC38 Cell Line.
Tumor Growth Curve
C57BL/6 mice were subcutaneously injected with tumor cells (5E5) in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b 2 where a and b were the long and short diameters of the tumor, respectively.
Tumor Cells Analysis
B-CAG-hCLDN18.2 MC38 Cells were subcutaneously implanted into C57BL/6 mice. Mice were divided into control and treatment groups (n=7), when the tumor size was approximately 150±50 mm3. hCLDN18.2 antibody showed substantially inhibitory effects in the mouse model. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.