B-CAG-hEPCAM EL4 Cell Line

Basic Information

Strain Name
EL4 Epcamtm1(hEPCAM)/Bcgen
Stock Number
310713
Common Name
B-CAG-hEPCAM EL4 Cell Line
Disease
Lymphoma
Species
Murine
Growth factors
Suspension
Culture medium
DMEM+10%FBS+1%L-Glutamin+1%NEAA+1%sodium pyruvate +1%HEPES+0.1% β-mercaptoethanol
Frozen
95%Complete medium+5%DMSO

Targeting Strategy

Details

Cell protein expression analysis

B-CAG-hEPCAM EL4 Cell Line were analyzed by flow cytometry.

Result: B-CAG-hEPCAM EL4 cells expressed human EPCAM protein. RT-PCR confirmed that mEPCAM protein was not expressed in EL4 cells.

Tumor growth curve

B-CAG-hEPCAM EL4 cells were subcutaneously implanted into the C57BL/6N mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Results: B-CAG-hEPCAM EL4 cells were able to establish tumor model steadily and track tumor volume effectively .

Tumor expression detection sample information

Protein expression analysis in tumor cells

Note:

FMO Control 1: Unstained mEpCAM-AF488 antibody;

FMO Control 2: Unstained hEpCAM APC antibody

B-CAG-hEPCAM EL4 tumor cells were transplanted into C57BL/6N mice, and a certain volume of tumor was used to detect the cell expression.

Results: hEPCAM was highly expressed on the surface of tumor cells.

Protein expression analysis in tumor cells

B-CAG-hEPCAM EL4 cells were subcutaneously transplanted into C57BL/6 mice, and a certain volume of tumor was taken to detect the EPCAM expression.

RESULTS: hEPCAM was highly expressed on the surface of tumor cells.

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