Basic Information

Common Name
Catalog Number
Cell Type/Disease
Colon Carcinoma
Mus musculus, C57BL/6

Targeting strategy

The exogenous promoter and human HVEM coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Hvem gene, resulting in a non-functional transcript.

Protein expression analysis

HVEM expression analysis in B-hHVEM MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hHVEM MC38 cultures were stained with species-specific anti-HVEM antibody. Mouse HVEM was detectable in wild-type MC38 cells. Human HVEM was detected on the surface of B-hHVEM MC38 cells but not wild-type MC38 cells. The 2-A5 clone of B-hHVEM MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hHVEM MC38 cells. B-hHVEM MC38 cells (5×105 and 1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.