Phenotypic analysis of B-CAG-hPD-L1 B16-F10 Cell Line
Flow cytometry analysis of human PD-L1 Protein. The hPD-L1, but not mPD-L1, protein was detected in B-CAG-hPD-L1 B16-F10 Cell Line.
Tumor Growth Curve
Five C57BL/6 mice were subcutaneously injected with MC38 tumor cells (1E5) with 0.1mL PBS in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b 2 where a and b were the long and short diameters of the tumor, respectively.