B-CAG-hPD-L1 (v2)/hCD47 MC38

Basic Information

Strain Name
MC38 Cd274tm1(hCD274)Cd47tm1(hCD47)/Bcgen
Stock Number
320714
Common Name
B-CAG-hPD-L1(v2)/hCD47 MC38
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Growth factors
Adherent
Culture medium
DMEM+10%FBS+1%L-Glutamine+1%NEAA+1%Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-hPD-L1(v2)/hCD47 MC38. The human full-length CD47 CDS region was inserted in the Cd47 exon2 of wild-type MC38. The human full-length CD274 CDS region was inserted in the Cd274 exon3 of wild-type MC38.

Details

Protein expression analysis

The PD-L1 and CD47 expression analysis in B-CAG-hPD-L1(v2)/hCD47 MC38 cells by flow cytometry. Single cells were collected from B-CAG-hPD-L1(v2)/hCD47 MC38 cells, and analyzed by flow cytometry with species-specific anti-PD-L1 and anti-CD47 antibody. Human PD-L1 and CD47 was detectable in B-CAG-hPD-L1(v2)/hCD47 MC38 cells.

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-hPD-L1(v2)/hCD47 MC38 cells. B-CAG-hPD-L1(v2)/hCD47 MC38 cells (5×105) were subcutaneously implanted into the B-hPD-L1/hSIRPA/hCD47 mice (n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-hPD-L1(v2)/hCD47 MC38 were able to establish tumor in vivo and can be used for efficacy study.

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