Basic Information

Strain name
C57BL/6-Mertktm2(MERTK)Gas6tm1(GAS6)/Bcgen
Common name
B-hALB MC38
Catalog number
321909
Aliases
HSA; PRO0883; PRO0903; PRO1341
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hALB MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Alb gene was replaced by human ALB coding sequence in B-hALB MC38 cells. Human ALB can be secreted by B-hALB MC38 cells.

Targeting strategy

Gene targeting strategy for B-hALB MC38 cells. The exogenous promoter and human ALB coding sequence was inserted to replace part of murine exon 1~2. The insertion disrupts the endogenous murine Alb gene, resulting in a non-functional transcript.

Protein expression analysis

ALB expression analysis in B-hALB MC38 cells by ELISA. (A) Mouse ALB was detected in the supernatant of B-Tg(mAlb) MC38 cells but not wild-type MC38 cells. It has been verified that MC38 cells do not express the mouse ALB. (B) Human ALB was detected in the supernatant of B-hALB MC38 cells but not wild-type MC38 cells. The 3-B01 clone of B-hALB MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hALB MC38 cells. B-hALB MC38 cells (1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hALB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hALB MC38 cells were subcutaneously transplanted into C57BL/6N mice (n=5), and on 35 days post inoculation, tumor cells were harvested and assessed for mouse and human ALB expression by ELISA. As shown, human ALB was highly expressed tumor homogenate. Therefore, B-hALB MC38 cells can be used for in vivo efficacy studies of novel ALB therapeutics.

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