Basic Information

Common Name
B-hAMHR2 MC38
Catalog number
311530
Aliases
AMHR, MISR2, MISRII, MRII
Disease
Colon carcinoma
Genotype
Homozygous
Culture properties
Adherent
Organism
Mus musculus, mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hAMHR2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The mouse AMHR2 gene was replaced by human AMHR2 coding sequence in B-hAMHR2 MC38 cells. Human AMHR2 is highly expressed on the surface of B-hAMHR2 MC38 cells.

Gene Targeting Strategy

Gene targeting strategy for B-hAMHR2 MC38 cells. The exogenous promoter and human AMHR2 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. This insertion disrupts the endogenous murine Amhr2 gene, resulting in a non-functional transcript.

Protein Expression Analysis

Pre-inoculation

AMHR2 expression analysis in humanized B-hAMHR2 MC38 cells. Single cell suspensions from wild-type MC38 and B-hAMHR2 MC38 (clone #1-A01) cultures were stained with an anti-human AMHR2 antibody and analyzed by flow cytometry. Human AMHR2 protein was detected on the surface of B-hAMHR2 MC38 cells compared to wild-type MC38 cells. The 1-A01 clone was used for in vivo experiments.

 

Post-inoculation

B-hAMHR2 MC38 cells were subcutaneously transplanted into wild-type C57BL/6 mice (n=5). Tumor cells were harvested on day 30 post inoculation and assessed for human AMHR2 expression by flow cytometry using an anti-human AMHR2 antibody. As shown, human AMHR2 protein was highly expressed on the surface of tumor cells, indicating B-hAMHR2 MC38 cells can be used for in vivo efficacy studies of novel AMHR2 therapeutics.

Tumor Growth Curve

Subcutaneous homograft tumor </b><b>growth of B-hAMHR2 MC38 cells

Subcutaneous homograft tumor growth of B-hAMHR2 MC38 cells. B-hAMHR2 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). (A) Average tumor volume ± SEM, and (B) body weight (Mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hAMHR2 MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies.

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