Basic Information

Common Name
B-hANXA2 MC38
Catalog Number
321942
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Aliases
P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Tissue types
Colon
Tissue
Colon
NCBI Gene ID
Application
B-hANXA2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Anxa2 gene was replaced by human ANXA2 coding sequence in B-hANXA2 MC38 cells. Human ANXA2 is highly expressed on the surface of B-hANXA2 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hANXA2 MC38 cells. The exogenous promoter and human ANXA2 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Anxa2 gene, resulting in a non-functional transcript.

 

Protein expression analysis

ANXA2 expression analysis in B-hANXA2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hANXA2 MC38 cultures were stained with anti-ANXA2 antibody. Human ANXA2 was detected on the surface of B-hANXA2 MC38 cells and MC38 cells,  as the antibody is crossly reactive with ANXA2 in human and mice. It has been verified that B-hANXA2 MC38 cells do not express mouse mRNA by RT-PCR. The 2-A01 clone of B-hANXA2 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hANXA2 MC38 cells. B-hANXA2 MC38 cells (1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diaANXA2er X short diaANXA2er2. As shown in panel A, B-hANXA2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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