Basic Information
Description
The mouse Cd19 gene was replaced by human CD19 coding sequence in B-hCD19-luc GFP A20 cells. Human CD19 is highly expressed on the surface this cell. And this cell expresses firefly luciferase as a marker.
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Targeting strategy
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Gene targeting strategy for B-hCD19-luc GFP A20 cells. The exogenous promoter, human CD19 coding sequence and luciferase was inserted to replace part of murine Cd19 gene in B-hCD19-luc GFP A20 cells. Mouse Cd19 transcript was disrupted, the protein no longer be expressed.
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Protein expression analysis
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CD19 expression analysis in B-hCD19-luc GFP A20 cells by flow cytometry. Single cell suspensions from wild-type A20 and B-hCD19-luc GFP A20 cultures were stained with anti-CD19 antibody. Human CD19 was detected on the surface of B-hCD19-luc GFP A20 cells but not wild-type A20 cells. And mouse CD19 was not detected on B-hCD19-luc GFP A20 cells.
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In vitro luciferase activity of B-hCD19-luc GFP A20 cells
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Luminescence signal intensity of B-hCD19-luc GFP A20 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-hCD19-luc GFP A20 cells have strong luminescence signal.
