Basic Information

Common Name
B-hCD24 MC38
Aliases
CD24,CD24A,PRO940
Catalog Number
311050
Cell Type/Disease
Colon carcinoma
Species/Strain
Mus musculus, C57BL/6
NCBI Gene ID

Application

B-hCD24 MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting Strategy

The exogenous promoter and human CD24 coding sequence was inserted to replace part of murine exon 1 and all of exon 2. The insertion disrupts the endogenous murine Cd24 gene, resulting in a non-functional transcript.

Protein Expression Analysis

Pre-inoculation

CD24 expression analysis in B-hCD24 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD24 MC38 cultures were stained with an anti-human CD24 antibody. Human CD24 was detected on the surface of B-hCD24 MC38 cells but not wild-type MC38 cells. The 1-F02 clone of B-hCD24 MC38 cells was used for in vivo experiments.

Post-inoculation

B-hCD24 MC38 cells were subcutaneously implanted into C57BL/6 mice (n=5). Tumor cells were harvested on day 34 post-inoculation and assessed for CD24 expression by flow cytometry using species-specific antibodies. As shown, human CD24 was highly expressed on the surface of humanized B-hCD24 MC38 tumor cells, indicating B-hCD24 MC38 cells can be used for in vivo efficacy studies of novel CD24 therapeutics.

Tumor Growth Curve and Body Weight Changes

Tumor Growth Curve and Body Weight Changes in C57BL/6 Mice

Subcutaneous homograft tumor growth of B-hCD24 MC38 cells. B-hCD24 MC38 cells (1×106) and wild-type MC38 cells (1×106) were subcutaneously implanted into C57BL/6 mice (female, 6-7-week-old, n=5). (A) Average tumor volume ± SEM and (B) body weight (mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hCD24 MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies.