Basic Information

Common Name
B-hCD36 MC38
Aliases
BDPLT10, CHDS7, FAT, GP3B, GP4,GPIV, PASIV, SCARB3
Catalog Number
311094
Cell Type/Disease
Colon carcinoma
Species/Strain
Mus musculus, C57BL/6
NCBI Gene ID

Description

The mouse Cd36 gene was replaced by the human CD36 coding sequence in B-hCD36 MC38 cells. Human CD36 is highly expressed on the surface of B-hCD36 MC38 cells.

Application

B-hCD36 MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting strategy

The exogenous promoter and human CD36 coding sequence was inserted to replace part of mouse Cd36. The insertion disrupts the endogenous murine Cd36 gene, resulting in a non-functional transcript.

Protein Expression Analysis

Pre-inoculation

CD36 expression analysis in B-hCD36 MC38 cells by flow cytometry. Single cell suspensions from B-hCD36 MC38 cultures were stained with an anti-human CD36 antibody. Human CD36 was detected on the surface of B-hCD36 MC38 cells but not wild-type MC38 cells. The 10-G12 clone of B-hCD36 MC38 cells was used for in vivo experiments.

Post-inoculation

B-hCD36 MC38 cells were subcutaneously implanted into B-hCD36 mice (n=5). Tumor cells were harvested on day 35 post inoculation and assessed for human CD36 expression by flow cytometry. As shown, human CD36 was highly expressed on the surface of humanized B-hCD36 MC38 tumor cells, indicating B-hCD36 MC38 cells can be used for in vivo efficacy studies of novel CD36 therapeutics. *Note: B-hCD36 MC38 cells can not establish tumors in wild-type C57BL/6 mice.

Tumor Growth Curve

Tumor Growth Curve & Body Weight Changes in Humanized Mice

Subcutaneous homograft tumor growth of B-hCD36 MC38 cells. B-hCD36 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into B-hCD36 mice (female, 7-week-old, n=5). (A) Average tumor volume ± SEM and (B) body weight (mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD36 MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies. *Note: B-hCD36 MC38 cells can not establish tumors in wild-type C57BL/6 mice.

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