Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD37 MC38 cells. The exogenous promoter and the CDS of human CD37 was inserted to replace part of murine exon 3 and exons 4-7. The insertion disrupts the endogenous murine CD37 gene, resulting in a non-functional transcript.
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Protein expression analysis
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CD37 expression analysis in B-hCD37 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD37 MC38 cultures were stained with species-specific anti-CD37 antibody. Human CD37 was detected on the surface of B-hCD37 MC38 cells but not wild-type MC38 cells. The 1-D11 clone of B-hCD37 MC38 cells was used for in vivo experiments.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-hCD37 MC38 cells. B-hCD37 MC38 cells (2×105, 5×105, 3×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD37 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
Subcutaneous homograft tumor growth of B-hCD37 MC38 cells. B-hCD37 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-hCD3E mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD37 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.