Basic Information
Description
Human CD39 is highly expressed on the surface of B-hCD39 MC38 cells
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Application
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B-hCD39 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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Target strategy
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The exogenous promoter and human CD39 coding sequence were inserted into the targeting vector used in the lentivirus system.
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Protein expression analysis
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CD39 expression analysis in B-hCD39 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD39 MC38 cultures were stained with species-specific anti-CD39 antibodies. Human CD39 was detected on the surface of B-hCD39 MC38 cells but not wild-type MC38 cells. The 1-F07 clone of B-hCD39 MC38 cells was used for in vivo experiments.
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Tumor growth curve
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Subcutaneous homograft tumor growth of B-hCD39 MC38 cells. B-hCD39 MC38 cells (5×105, 1×106, 5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into B-hPD-1/hCD39 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Bodyweight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD39 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
