Basic Information
Description
The mouse Cdh17 gene was replaced by human CDH17 coding sequence in B-hCDH17 MC38 cells. Human CDH17 is highly expressed on the surface of B-hCDH17 MC38 cells.
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Targeting strategy
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Gene targeting strategy for B-hCDH17 MC38 cells. The exogenous promoter and human CDH17 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Cdh17 gene, resulting in a non-functional transcript.
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Protein expression analysis
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CDH17 expression analysis in B-hCDH17 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCDH17 MC38 cultures were stained with anti-CDH17 antibody. Human CDH17 was detected on the surface of B-hCDH17 MC38 cells but not wild-type MC38 cells.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-hCDH17 MC38 cells. B-hCDH17 MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=6 or 8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCDH17 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
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Tumor growth curve of individual mice
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B-hCDH17 MC38 tumor growth of individual mice. B-hCDH17 MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=6 or 8). As shown in panel, B-hCDH17 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
