Basic Information

Common name
B-hGPNMB MC38
Catalog number
322002
Aliases
NMB; HGFIN; PLCA3
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hGPNMB MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Gpnmb gene was replaced by human GPNMB coding sequence in B-hGPNMB MC38 cells. Human GPNMB is highly expressed on the surface of B-hGPNMB MC38 cells.

Targeting strategy

Gene targeting strategy for B-hGPNMB MC38 cells. The exogenous promoter and human GPNMB coding sequence was inserted to replace part of murine exon 2-3. The insertion disrupts the endogenous murine Gpnmb gene, resulting in a non-functional transcript.

Protein expression analysis

GPNMB expression analysis in B-hGPNMB MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hGPNMB MC38 cultures were stained with species-specific anti-GPNMB antibody. Human GPNMB was detected on the surface of B-hGPNMB MC38 cells but not wild-type MC38 cells. The 3-B12 clone of B-hGPNMB MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hGPNMB MC38 cells. B-hGPNMB MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGPNMB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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