Basic Information

Common Name
B-hHRH3 MC38
Catalog Number
321956
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Aliases
HH3R; GPCR97
Tissue types
Colon
Tissue
Colon
NCBI Gene ID
Application
B-hHRH3 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Hrh3 gene was replaced by human HRH3 coding sequence in B-hHRH3 MC38 cells. Human HRH3 is highly expressed on the surface of B-hHRH3 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hHRH3 MC38 cells. The exogenous promoter and human HRH3 coding sequence was inserted to replace part of murine exon 1 and all of exons 2-3. The insertion disrupts the endogenous murine Hrh3 gene, resulting in a non-functional transcript.

 

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hHRH3 MC38 cells. B-hHRH3 MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 5-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHRH3 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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