Basic Information

Common Name
B-hLRRC15 MC38
Catalog Number
321867
Alias
LIB
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue
Colon
Application
B-hLRRC15 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Lrrc15 gene was replaced by human LRRC15 coding sequence in B-hLRRC15 MC38 cells. Human LRRC15 is highly expressed on the surface of B-hLRRC15 MC38 cells. B-hLRRC15 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Gene targeting strategy

Gene targeting strategy for B-hLRRC15 MC38 cells. The exogenous promoter and human LRRC15 coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Lrrc15 gene, resulting in a non-functional transcript.

Protein expression analysis

LRRC15 expression analysis in B-hLRRC15 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hLRRC15 MC38 cultures were stained with an anti-human LRRC15 antibody and analyzed by flow cytometry. Human LRRC15 was detected on the surface of B-hLRRC15 MC38 cells but not on wild-type MC38 cells. Clone 2-C04 was used for in vivo experiments.

Tumor growth curve and body weight changes

Subcutaneous homograft tumor growth of B-hLRRC15 MC38 cells. B-hLRRC15 MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-9-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLRRC15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis - post-inoculation

B-hLRRC15 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6), 28 days post-inoculation, tumor cells were harvested and assessed for human LRRC15 expression by flow cytometry. As shown, human LRRC15 was highly expressed on the surface of tumor cells. Therefore, B-hLRRC15 MC38 cells can be used for in vivo efficacy studies testing novel LRRC15 therapeutics.