Basic Information

Common name
B-hLRRN1 MC38
Catalog number
322195
Aliases
NLRR-1; FIGLER3
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hLRRN1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The mouse Lrrn1 gene was replaced by human LRRN1 coding sequence in B-hLRRN1 MC38 cells. Human LRRN1 is highly expressed on the surface of B-hLRRN1 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hLRRN1 MC38 cells. The exogenous promoter and human LRRN1 coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Lrrn1 gene, resulting in a non-functional transcript.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hLRRN1 MC38 cells. B-hLRRN1 MC38 cells (5×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (male, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLRRN1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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