Basic Information

Common name
B-hMSLN ID8
Catalog number
321823
Aliases
MPF, SMRP
Disease
Ovarian Cancer
Organism
Mouse
Strain
C57BL/6
Tissue types
Ovary
Tissue
Ovary
Application
B-hMSLN ID8 cells have the capability to establish tumors in C57BL/6N mice in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The human MSLN coding sequences were inserted to mouse Msln gene exon 2 in B-hMSLN ID8 cells, so that the mouse MSLN was not expressed anymore, human MSLN was highly expressed on the surface of B-hMSLN ID8 cells.

Targeting strategy

Gene targeting strategy for B-hMSLN ID8 cells. The exogenous promoter and human MSLN CDS was inserted to mouse Msln gene exon 2 in B-hMSLN ID8 cells.

Protein expression analysis

MSLN expression analysis in B-hMSLN ID8 cells by flow cytometry. Single cell suspensions from B-hMSLN ID8 cultures were stained with species-specific anti-MSLN antibody. Human MSLN were detected on the surface of B-hMSLN ID8 cells. The 2-A11 clone of B-hMSLN ID8 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hMSLN ID8 cells. B-hMSLN ID8 cells (5×106) and wild-type ID8 cells (1×106) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hMSLN ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hMSLN ID8 cells were subcutaneously transplanted into C57BL/6 mice (n=8), and on 21 days post inoculation, tumor cells were harvested and assessed for human MSLN expression by flow cytometry. As shown, human MSLN was highly expressed on the surface of tumor cells. Therefore, B-hMSLN ID8 cells can be used for in vivo efficacy studies of novel MSLN therapeutics.