Basic Information

Common name
B-hMUC16 ID8
Catalog number
321841
Aliases
CA125
Disease
Ovarian Cancer
Organism
Mouse
Strain
C57BL/6
Tissue
Ovary
Application
B-hMUC16 ID8 cells have the capability to establish tumors in C57BL/6 mice in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The 13810-14507 aa of human MUC16 coding sequences was inserted to mouse Muc16 gene locus in B-hMUC16 ID8 cells, truncated human MUC16 was highly expressed on the surface of B-hMUC16 ID8 cells. It is recommended to do binding experiments before use.

Gene targeting strategy

Gene targeting strategy for B-hMUC16 ID8 cells. The exogenous promoter and 13810-14507aa human MUC16 CDS was inserted to mouse Muc16 gene in B-hMUC16 ID8 cells.

Protein expression analysis

MUC16 protein expression analysis in B-hMUC16 ID8 cells. Single cell suspensions from B-hMUC16 ID8 cultures were stained with species-specific anti-MUC16 antibodies. Human MUC16 protein was detected on the surface of B-hMUC16 ID8 cells. The 1-F11 clone of B-hMUC16 ID8 cells was used for in vivo experiments.

Tumor growth curve in wild-type mice

Subcutaneous homograft tumor growth of B-hMUC16 ID8 cells. B-hMUC16 ID8 cells (5×105) and wild-type ID8 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A)  Body weight (Mean± SEM). (B) Average tumor volume ± SEM. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC16 ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

B-hMUC16 ID8 tumor cell growth in individual mice. B-hMUC16 ID8 cells (5×105) and wild-type ID8 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6).

Tumor volume and weight measurements

Protein expression analysis post-inoculation

(A) B-hMUC16 ID8 tumor cells were harvested and assessed for human MUC16 expression by flow cytometry 28 days post inoculation. As shown, human MUC16 was expressed on the surface of tumor cells. (B) ID8 cells and B-hMUC16 ID8 #1-F11 cells were assessed for human MUC16 expression by flow cytometry.