Basic Information

Common name
B-hOLFML3 MC38
Catalog number
321865
Aliases
OLF44; HNOEL-iso
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hOLFML3 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The mouse Olfml3 gene was replaced by human OLFML3 coding sequence in B-hOLFML3 MC38 cells. Human OLFML3 is highly expressed on the surface of B-hOLFML3 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hOLFML3 MC38 cells. The exogenous promoter and human OLFML3 coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Olfml3 gene, resulting in a non-functional transcript.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hOLFML3 MC38 cells. B-hOLFML3 MC38 cells (1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hOLFML3 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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