Basic Information
Description
The mouse Pdl1 gene and Cd47 gene were replaced by human PD-L1 and CD47 coding sequence in B-hPD-L1 plus/hCD47 MC38 cells. Human PD-L1 and CD47 are highly expressed on the surface of B-hPD-L1 plus/hCD47 MC38 cells.
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Targeting strategy
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- The exogenous promoter and human PD-L1 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.
- The exogenous promoter and human CD47 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd47 gene, resulting in a non-functional transcript.
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Protein expression analysis
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PD-L1 and CD47 expression analysis in B-hPD-L1 plus/hCD47 MC38 cells by flow cytometry. Single cell suspensions from B-hPD-L1 plus/hCD47 MC38 cultures were stained with species-specific anti-PD-L1 and anti-CD47 antibodies. Human PD-L1 and CD47 were detected on the surface of B-hPD-L1 plus/hCD47 MC38 cells. The 3-D07 clone of B-hPD-L1 plus/hCD47 MC38 cells was used for in vivo experiments.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-hPD-L1 plus/hCD47 MC38 cells. B-hPD-L1 plus/hCD47 MC38 cells (5×105) were subcutaneously implanted into B-hPD-L1/hSIRPA/hCD47 mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 plus/hCD47 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.