B-hTNFR2 MC38 Cell Line

Basic Information

Strain Name
MC38 Tnfrsf1btm1(hTNFRSF1B)/Bcgen
Stock Number
310723
Common Name
B-hTNFR2 MC38 Cell Line
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Growth factors
Adherent
Culture medium
DMEM+10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO

Targeting Strategy

Details

Cell protein expression analysis

B-hTNFR2 MC38 Cell Line were analyzed by flow cytometry.

Result: B-hTNFR2 MC38 Cell Line expressed human TNFR2 protein, but not mouse TNFR2 protein.

Tumor growth curve

B-hTNFR2 MC38 cells were subcutaneously implanted into the C57BL/6N mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Results: B-hTNFR2 MC38 cells were able to establish tumor model steadily and can effectively track tumor volume.

Tumor expression detection sample information

Protein expression analysis in tumor cells

Note:

FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;

FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.

Protein expression analysis in tumor cells

Note:

FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;

FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.

B-hTNFR2 MC38 was transplanted into C57BL/6N mice(n=5), and on 27 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells.

Results: hTNFR2 was highly expressed on the surface of tumor cells.

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