Basic Information

Strain Name
MC38 Tnfrsf1btm1(hTNFRSF1B)/Bcgen
Stock Number
310723
Common Name
B-hTNFR2 MC38
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Growth factors
Adherent
Culture medium
DMEM+10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Details

Cell protein expression analysis

B-hTNFR2 MC38 Cell Line were analyzed by flow cytometry.

Result: B-hTNFR2 MC38 Cell Line expressed human TNFR2 protein, but not mouse TNFR2 protein.

Tumor growth curve

B-hTNFR2 MC38 cells were subcutaneously implanted into the C57BL/6N mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Results: B-hTNFR2 MC38 cells were able to establish tumor model steadily and can effectively track tumor volume.

Tumor expression detection sample information

Protein expression analysis in tumor cells

Note:

FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;

FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.

Protein expression analysis in tumor cells

Note:

FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;

FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.

B-hTNFR2 MC38 was transplanted into C57BL/6N mice(n=5), and on 27 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells.

Results: hTNFR2 was highly expressed on the surface of tumor cells.

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