Basic Information

Common Name
B-hTPBG B16-F10
Aliases
5T4, 5T4AG, M6P1, WAIF1
Catalog Number
311265
Cell Type/Disease
Melanoma
Species/Strain
Mus musculus, C57BL/6
NCBI Gene ID

Application

B-hTPBG B16-F10 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting Strategy

The exogenous promoter and human TPBG coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Tpbg gene, resulting in a non-functional transcript.

Protein Expression Analysis

Pre-inoculation

TPBG expression analysis in B-hTPBG B16-F10 cells by flow cytometry. Single cell suspensions from B-hTPBG B16-F10 cultures were stained with an anti-human TPBG antibody. Human TPBG was detected on the surface of B-hTPBG B16-F10 cells but not wild-type B16-F10 cells. The 2-E11 clone of B-hTPBG B16-F10 cells was used for in vivo experiments.

Post-inoculation

B-hTPBG B16-F10 cells were subcutaneously transplanted into C57BL/6 mice (n=5). Tumor cells were harvested on day 19 post inoculation and assessed for human TPBG expression by flow cytometry. As shown, human TPBG was highly expressed on the surface of humanized B-hTPBG B16-F10 tumor cells, indicating B-hTPBG B16-F10 cells can be used for in vivo efficacy studies of novel TPBG therapeutics.

 

B-hTPBG B16-F10 cells were subcutaneously implanted into B-hCD40 mice and B-hPD-1/hCD40 mice (n=7). Tumor cells were harvested on day 14 post inoculation and assessed for human TPBG expression by flow cytometry. As shown, human TPBG was highly expressed on the surface of humanized B-hTPBG B16-F10 tumor cells, indicating B-hTPBG B16-F10 cells can be used for in vivo efficacy studies of novel TPBG therapeutics.

Tumor Growth Curve & Body Weight Changes

Tumor Growth Curve & Body Weight Changes in C57BL/6 Mice

Subcutaneous homograft tumor growth of B-hTPBG B16-F10 cells. B-hTPBG B16-F10 cells (2×105) and wild-type B16-F10 cells (2×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). (A) Average tumor volume ± SEM and (B) body weight (Mean± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTPBG B16-F10 cells were able to establish tumors in vivo, which can be used for efficacy studies.

Tumor Growth Curve & Body Weight Changes in Humanized Mice

Subcutaneous homograft tumor growth of B-hTPBG B16-F10 cells. B-hTPBG B16-F10 cells (2×105) were subcutaneously implanted into B-hCD40 mice and B-hPD-1/hCD40 mice (female, 7-week-old, n=7). (A) Average tumor volume ± SEM and (B) body weight (Mean± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTPBG B16-F10 cells were able to establish tumors in vivo, which can be used for efficacy studies.