Basic Information

Common name
B-luc A20
Catalog number
311118
Aliases
Gt(ROSA)26Sor
Disease
Reticulum cell sarcoma
Organism
Mouse
Strain
BALB/cAnN
Tissue types
Lymphoma
Tissue
B lymphocyte
Application
B-Luc A20 cell line can be used to evaluate anti-cancer drugs in reticulum cell sarcoma in vivo.

Description

This B-Luc A20 cell line expresses firefly luciferase as a marker of A20 cells. Fluorescence and luminescence can be observed in B-Luc A20 cells.

Targeting Strategy

Gene targeting strategy for B-Luc A20 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.

In vitro Luciferase Activity of B-Luc A20 cells

Luminescence signal intensity of B-Luc A20 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc A20 cells have a strong luminescence signal. Clone No. :2-A10.

Quantitative Imaging Data & Body Weight Changes

Tumor growth and in vivo imaging of B-Luc A20 cells. B-Luc A20 cells (5×105) were injected into the tail vein of wild-type BALB/c mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc A20 cells can be used for in vivo efficacy evaluation.

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