Basic Information

Cell Line Name
B16-F10 Gt(ROSA)26Sortm1(luciferase)/Bcgen
Common Name
B-luc B16-F10 
Catalog Number
310680
Cell Type/Disease
Melanoma
Culture Properties
Adherent
Resistance
Puromycin, 0.2 μg/ml
Medium
DMEM+10%FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Cryopreservation Medium
90% Complete Medium+10% DMSO
Detecting Instrument
Bright-GloTM luciferase Assay System (Promega, Cat E2610)

Targeting Strategy

Gene targeting strategy for B-luc B16-F10 cells. In addition to the CAG promoter, the luciferase cDNA sequence was inserted in exon 1 of the ROSA26 locus in wild-type B16-F10.

Genotype Identification

Phenotypic Analysis

Luminescence signal intensity of B-luc B16-F10 cells. B-luc B16-F10 cells displayed strong luminescence signal intensity compared to the control group, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).