Basic Information

Cell Line Name
Common Name
B-luc EL4
Related gene
Catalog Number
Cell Type/Disease
Culture Properties
DMEM+10%FBS+1%L-Glutamin+1%NEAA+1%sodium pyruvate +1%HEPES+0.1% β-mercaptoethanol 
Cryopreservation Medium
95% Complete Medium+5% DMSO
Detecting  Instrument
Bright-GloTM luciferase Assay System (Promega, Cat E2610)

Targeting Strategy

Gene targeting strategy for B-luc EL4 cells. The luciferase sequence was inserted in exon 1 of the ROSA26 locus in wild-type EL4 cells. 

Genotype Identification

Phenotypic Analysis

Luminescence signal intensity of B-luc EL4 cells. B-luc EL4 cells displayed strong luminescence signal intensity compared to wild-type EL4 cells, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).

In Vivo Imaging Analysis

Tumor growth and in vivo imaging of B-luc EL4 cells. B-luc EL4 cells (2×105) were injected by tail vein into C57BL/6 mice (female, 7-8 week-old, n=5). (A) Imaging was performed on days 0, 3, 6, 10, 13, 17, 20, 24 and 27 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc EL4 cells can be used for in vivo efficacy evaluation.