Basic Information
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Targeting Strategy
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Gene targeting strategy for B-luc EL4 cells. The luciferase sequence was inserted in exon 1 of the ROSA26 locus in wild-type EL4 cells.
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Genotype Identification
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Phenotypic Analysis
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Luminescence signal intensity of B-luc EL4 cells. B-luc EL4 cells displayed strong luminescence signal intensity compared to wild-type EL4 cells, as detected by the Bright-GloTM luciferase Assay System (Promega, Cat E2610).
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In Vivo Imaging Analysis
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Tumor growth and in vivo imaging of B-luc EL4 cells. B-luc EL4 cells (2×105) were injected by tail vein into C57BL/6 mice (female, 7-8 week-old, n=5). (A) Imaging was performed on days 0, 3, 6, 10, 13, 17, 20, 24 and 27 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc EL4 cells can be used for in vivo efficacy evaluation.
