Basic Information
Description
This B-Luc LLC1 cell line expresses firefly luciferase as a marker of LLC1 cells. Luminescence can be observed in B-Luc LLC1 cells.
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Targeting strategy
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Gene targeting strategy for B-Luc LLC1 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.
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In vitro luciferase activity of B-Luc LLC1 cells
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Luminescence signal intensity of B-Luc LLC1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc LLC1 cells have a strong luminescence signal. The 1-H04 clone of B-Luc LLC1 cells can be used in vivo experiments.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-Luc LLC1 cells. B-Luc LLC1 cells (1×105 and 2×105) and wild-type LLC1 cells (1×105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Luc LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.
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Quantitative imaging data & Body weight changes
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Tumor growth and in vivo imaging of B-Luc LLC1 cells. B-Luc LLC1 cells (1×105 and 2×105) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc LLC1 cells can be used for in vivo efficacy evaluation.
