Basic Information

Common Name
B-Tg(hCCL1) MC38
Catalog number
321855
Disease
Colon carcinoma
Organism
Mus musculus, mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-Tg(hCCL1) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The exogenous promoter and human CCL1 coding sequence were inserted into the mouse genome randomly. Human CCL1 can be secreted by B-Tg(hCCL1) MC38 cells, and drive more T cells into the tumor microenvironment in C57BL/6 mice bearing MC38 cells.

Gene Targeting Strategy

Gene targeting strategy for B-Tg(hCCL1) MC38 cells. The exogenous promoter and human CCL1 coding sequence were inserted into the mouse genome randomly.

Protein Expression Analysis

Pre-inoculation

Human CCL1 expression analysis in B-Tg(hCCL1) MC38 cells. Human CCL1 protein was detected in the supernatant of B-Tg(hCCL1) MC38 cells compared to wild-type MC38 cells using a species-specific ELISA kit. The 2-C12 clone of B-Tg(hCCL1) MC38 cells was used for in vivo experiments.

 

Post-inoculation

Tumor cells were harvested and assessed for mouse and human CCL1 expression. Using species-specific ELISA kit, murine and human CCL1 were highly expressed in the tumor homogenate. Data was shown as Mean ± SEM and analyzed using one-way ANOVA followed Dunnett test compared with G1.

Tumor Growth Curve

Subcutaneous homograft tumor growth of B-Tg(hCCL1) MC38 cells. B-Tg(hCCL1) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). (A) Average tumor volume ± SEM, and (B) body weight (Mean± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-Tg(hCCL1) MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies.

 

Subcutaneous homograft tumor growth of B-Tg(hCCL1) MC38 cells. B-Tg(hCCL1) MC38 (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into humanized B-hCCR8 mice (female, 8-week-old, n=7). (A) Average tumor volume ± SEM, and (B) body weight (Mean± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-Tg(hCCL1) MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies.

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