Basic Information

Strain name
NOD.CB17-PrkdcscidBcgenIl2rgtm1BcgenB2mtm1(B2M/HLA-A2.1)Bcgen Fcgrttm1(B2m/Fcgrt)Bcgen/Bcgen
Common Name
B-NDG hB2M/HLA-A2.1 mice plus
Background
B-NDG mice
Catalog number
112449
Aliases
B2m: Ly-m11, beta2-m, beta2m HLA-A: HLAA Fcgrt : Fc receptor, IgG, alpha chain transporter,as known as Fcrn

Targeting strategy

Gene targeting strategy for B-NDG HLA-2.1 mice. The B2m gene (Exon1 to Exon3) of mouse was replaced by the sequence encompassing the human B2M CDS and HLA-A2.1 gene in B-NDG HLA-2.1 mice. The mouse B2m gene was knocked out while a fused gene composed of mouse B2m and Fcgrt gene was inserted after the signal peptide sequence of mouse Fcgrt gene in B-NDG B2m KO mice plus. The B-NDG HLA-2.1 mice were mated with B-NDG B2m KO mice plus to obtain a new mouse strain named B-NDG hB2M/HLA-A2.1 mice plus.

Determination of plasma concentrations of human IgG in different mice

Pharmacokinetic characteristic of B-NDG hB2M/HLA-A2.1 mice plus has no difference compared with B-NDG mice.

B-NDG mice, B-NDG HLA-A2.1 mice, B-NDG hB2M/HLA-A2.1 mice plus were treated with YH001 (anti-human CTLA4 antibody) (n=4). Blood samples were collected at different time point for the PK assay. The results showed that the PK results of B-NDG hB2M/HLA-A2.1 mice plus group was similar to that in B-NDG mice, and the drug concentration of B-NDG HLA-A2.1 mice group could not be measured at the time point 4 days later. Results indicated that when compared with B-NDG HLA-A2.1 mice, the B-NDG hB2M/HLA-A2.1 mice plus can significantly extend the half-life of antibody drugs in mice.

Summary

Determination of plasma concentrations of human IgG in different mice.

Compared with B-NDG HLA-A2.1 mice, the B-NDG hB2M/HLA-A2.1 mice plus can significantly extend the half-life of antibody drugs in mice.

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