Basic Information
Description
Immunodeficient B-NDG mice were generated by deleting the IL2rg gene from NOD-scid mice. This mouse model has the highest degree of immunodeficiency and is most suitable for engraftment and growth of human tumor cells or tissues.
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Targeting strategy
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Gene targeting strategy for B-NDG Hc mice. The exon 6 of mouse Hc gene was insert by “TA” between “AC” in B-NDG Hc mice.
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Measurement of C5 in Serum
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C5 expression analysis in homozygous B-NDG Hc mice by ELISA. Serum were collected from C57BL/6, B-NDG mice and homozygote B-NDG Hc mice, and analyzed by ELISA with C5 ELISA kit. Mouse C5 was detectable in C57BL/6 mice and B-NDG Hc mice. but not B-NDG mice.
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Measurement of total complement hemolytic activity in Serum
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Hemolytic capacity analysis in homozygous B-NDG Hc mice. Representative image demonstrating level of lysis of antibody-coated SRBC following incubation with diluted serum from human, C57BL6, B-NDG, or B-NDG Hc mice. Serum from human were used as positive complement control. Compared with B-NDG, the hemolytic ability of B-NDG Hc was significantly enhanced. The order of hemolytic capacity: B-NDG Hc ≥ C57BL/6 > B-NDG.
