Basic Information

Strain name
NOD.CB17-PrkdcscidIl2rgtm1Hctm1(c645insTA)/Bcgen
Common Name
B-NDG Hc mice
Background
B-NDG
Catalog Number
110605
Aliases
C5, C5a, He, Hfib, Hfib2
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-NDG Hc mice. The exon 6 of mouse Hc gene was insert by “TA” between “AC” in B-NDG Hc mice.

Measurement of C5 in Serum

C5 expression analysis in homozygous B-NDG Hc mice by ELISA. Serum were collected from C57BL/6, B-NDG mice and homozygote B-NDG Hc mice, and analyzed by ELISA with C5 ELISA  kit. Mouse C5 was detectable in C57BL/6 mice and B-NDG Hc mice. but not B-NDG mice.

Measurement of  total complement hemolytic activity in Serum

Hemolytic capacity analysis in homozygous B-NDG Hc mice. Representative image demonstrating level of lysis of antibody-coated SRBC following incubation with diluted serum from human, C57BL6, B-NDG, or B-NDG Hc mice. Serum from human were used as positive complement control. Compared with B-NDG, the hemolytic ability of B-NDG Hc was significantly enhanced. The order of hemolytic capacity: B-NDG Hc ≥ C57BL/6 > B-NDG.

Summary

Measurement of C5 in Serum:

Mouse C5 was detectable in C57BL/6 mice and B-NDG Hc mice. but not B-NDG mice.

Measurement of  total complement hemolytic activity in Serum:

Compared with B-NDG, the hemolytic ability of B-NDG Hc was significantly enhanced.