Basic Information

Strain name
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Csf1tm1(CSF1)Bcgen/Bcgen
Common name
B-NDG hCSF1 mice
Background
B-NDG
Catalog number
110591
Aliases
CSF-1, MCSF, PG-M-CSF
NCBI gene ID
1435

Targeting strategy

Gene targeting strategy for B-NDG hCSF1 mice. The full sequences of mouse Csf1 genes except the UTRs were respectively replaced with the coding sequences (CDS) of human CSF1 gene.

Protein expression analysis in spleen

Strain specific CSF1 expression analysis in B-NDG mice and homozygous B-NDG hCSF1 mice by ELISA. Spleens were collected from B-NDG mice and homozygous B-NDG hCSF1 mice stimulated with 100 μg/mL LPS in vivo for 2 hrs (200 μL/mouse). Grinding supernatant from the spleens was analyzed by ELISA. Mouse CSF1 was only detectable in B-NDG mice. Human CSF1 was exclusively detectable in homozygous B-NDG hCSF1 mice. Values are expressed as mean ± SEM.

Back to top
WordPress Double Opt-in by Forge12