Basic Information
Strain name
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Csf1tm1(CSF1)Bcgen/Bcgen
Common name
B-NDG hCSF1 mice
Background
B-NDG
Catalog number
110591
Aliases
CSF-1, MCSF, PG-M-CSF
NCBI gene ID
1435
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Targeting strategy
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Gene targeting strategy for B-NDG hCSF1 mice. The full sequences of mouse Csf1 genes except the UTRs were respectively replaced with the coding sequences (CDS) of human CSF1 gene.
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Protein expression analysis in spleen
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Strain specific CSF1 expression analysis in B-NDG mice and homozygous B-NDG hCSF1 mice by ELISA. Spleens were collected from B-NDG mice and homozygous B-NDG hCSF1 mice stimulated with 100 μg/mL LPS in vivo for 2 hrs (200 μL/mouse). Grinding supernatant from the spleens was analyzed by ELISA. Mouse CSF1 was only detectable in B-NDG mice. Human CSF1 was exclusively detectable in homozygous B-NDG hCSF1 mice. Values are expressed as mean ± SEM.