Basic Information
Strain name
NOD.CB17-PrkdcscidIl2rgtm1Il7tm1(IL7)Il15tm1(IL15)/Bcgen
Common Name
B-NDG hIL7/hIL15 mice
Background
B-NDG
Catalog number
120612
NCBI Gene ID
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Gene targeting strategy
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Gene targeting strategy for B-NDG hIL7/hIL15 mice. The CDS region of the human IL7 gene was inserted after exon 1 of the mouse Il7 gene in B-NDG hIL7/hIL15 mice. The CDS region of the human IL15 gene was inserted after the 5′UTR of the mouse Il15 gene in B-NDG hIL7/hIL15 mice.
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Protein expression analysis
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Human IL7 and IL15 protein expression analysis in B-NDG hIL7/hIL15 mice. Serum was collected from immunodeficient B-NDG and homozygous B-NDG hIL7/hIL15 (H/H) mice stimulated with poly(I:C) in vivo, and analyzed using species-specific hIL7/IL15 ELISA kits. Human IL7 and IL15 proteins were exclusively detected in B-NDG hIL7/ hIL15 mice.
