Basic Information
Strain name
NOD.CB17-PrkdcscidIl2rgtm1Il7tm1(IL7)Il15tm1(IL15)/Bcgen
Common Name
B-NDG hIL7/hIL15 mice
Background
B-NDG
Catalog number
120612
NCBI Gene ID
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Gene Targeting Strategy
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Gene targeting strategy for B-NDG hIL7/hIL15 mice. The CDS region of the human IL7 gene was inserted after exon 1 of the mouse Il7 gene in B-NDG hIL7/hIL15 mice. The CDS region of the human IL15 gene was inserted after the 5′UTR of the mouse Il15 gene in B-NDG hIL7/hIL15 mice.
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Protein Expression Analysis
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Strain specific IL7/IL15 expression analysis in homozygous B-NDG hIL7/hIL15 mice by ELISA. Serum were collected from WT and homozygous B-NDG hIL7/hIL15 (H/H) mice stimulated with poly(I:C) in vivo, and analyzed by ELISA with species-specific hIL7/IL15 ELISA kit. Human IL7/IL15 were exclusively detectable in homozygous B-NDG hIL7/ hIL15 but not WT B-NDG mice.
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Summary
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Protein expression analysis:
- Human IL7/IL15 was exclusively detectable in homozygous B-NDG hIL7/ hIL15 but not WT B-NDG mice
