B-NDG KitW-v mice

Basic Information

Strain Name
B-NDG-Kittm1(w-v)/Bcgen
Common Name
B-NDG Kitw-v mice
Background
B-NDG
Catalog number
110588
Related Genes
Kit (KIT proto-oncogene receptor tyrosine kinase)

Description

Kit (KIT proto-oncogene receptor tyrosine kinase) , which is expressed on hematopoietic stem and progenitor cells (HSPCs), functions as an essential regulator of the interaction between macrophages, monocytes and NK cells and their niches by binding to its ligand, stem cell factor (SCF).

For successful full-lineage engraftment, human HSPCs May need to find suitable niches within the mouse bone Marrow.It was shown that immunodeficient mice with Loss-of-function Kit mutations or treated with a neutralizing anti-mouse KIT antibody accepted donor mouse HSCs Without irradiation preconditioning that may be critical for the depletion of host HSCs to open their niches.

Biocytogen established a KitWv mutation on the background of B-NDG mice (B: Biocytogen; N: NOD background; D: DANPK (Prkdc) null; G: Il2rg knockout.), Is a powerful tool for human immune system reconstituted.

Targeting strategy

Details

B-NDG Kitw-v Mice are Well Suited for Human Immune System Engraftment

CD34+ constitution standard:hCD45%≥25%

 

Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG Kitw-v mice show a significantly higher percentage of human CD45+ cells and of multi-lineage cells,including CD3+ T cells and CD19+ B cells. Our results suggest that human B, NK and T cells in reconstituted B-NDG mice were successfully propagated.

B-NDG Kitw-v Mice Are Well Suited for Human Immune System Engraftment

CD34+ constitution standard:hCD45%≥25%

 

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