Basic Information
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Description
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- The Abcb4 gene encodes a transmembrane transporter protein that moves phospholipids from the inner to the outer leaflet of the canalicular membrane in liver cells. This process is crucial for forming healthy bile and helps protect liver cells from toxic damage caused by bile salts. When the function of ABCB4 is impaired or absent, it can lead to reduced secretion of phospholipids, increased concentration of bile salts, and potentially induce hepatic pathologies.
- Gene editing strategy: The exons 2~28 of mouse Abcb4 gene were depleted in B-Abcb4 KO mice(C).
- mRNA expression analysis: Mouse Abcb4 mRNA was detectable only in wild-type BALB/C mice, but not in B-Abcb4 KO mice(C) (-/-).
- H&E staining of B-Abcb4 KO mice(C): There were no abnormal changes in the liver of wild-type BALB/C mice, but in the liver of B-Abcb4 KO mice (-/-), proliferation of intrahepatic bile ducts, thickening of the duct walls, and a small amount of inflammatory cell infiltration could be observed.
- Sirius red staining of B-Abcb4 KO mice(C): Compared to wild-type BALB/C mice, B-Abcb4 KO mice (C) (-/-) exhibited a significant increase in the positive signal area for collagen fibers.
- Application: This product is used for pharmacodynamics evaluation on diseases such as progressive cholestasis, cholestatic hepatitis, liver fibrosis and liver cancer.
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Targeting Strategy
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Gene targeting strategy for B-Abcb4 KO mice(C).
The exons 2~28 of mouse Abcb4 gene were depleted in B-Abcb4 KO mice(C).
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mRNA expression analysis in B-Abcb4 KO mice(C)
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Mouse Abcb4 mRNA expression in wild-type BALB/C mice and B-Abcb4 KO mice(C) by RT-PCR. Liver and lung RNA were isolated from wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcb4 primers. Mouse Abcb4 mRNA was detectable only in wild-type BALB/C mice, but not in B-Abcb4 KO mice(C) (-/-).
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H&E staining of B-Abcb4 KO mice(C)
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H&E staining of B-Abcb4 KO mice(C). Liver tissues from BALB/C mice and B-Abcb4 KO mice(C) (n=3, Male, 6 week-old; n=2, Female, 6 week-old) were collected and analyzed for H&E staining. There were no abnormal changes in the liver of wild-type BALB/C mice, but in the liver of B-Abcb4 KO mice, proliferation of intrahepatic bile ducts, thickening of the duct walls, and a small amount of inflammatory cell infiltration could be observed. (a) Bile ducts (b) Inflammatory
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Sirius red staining of B-Abcb4 KO mice(C)
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Sirius red staining of B-Abcb4 KO mice(C). (A) Representative images of Sirius red staining showing liver fibrosis in wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-) (n=3, Male, 6 week-old; n=2, Female, 6 week-old). (B) Statistic data of Sirius red staining. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.
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Biochemistry analysis
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Biochemical test of B-Abcb4 KO mice(C). Values are expressed as mean ± SEM. N=6 mice per group. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
