Basic Information
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Description
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- LDLR, or Low-Density Lipoprotein Receptor, is a critical cell surface receptor involved in cholesterol metabolism. It facilitates the uptake of LDL particles, which contain high levels of cholesterol, by binding to apolipoproteins B-100 and E.
- Expression the LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
- Gene editing strategy: the exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
- Application: This product is used for pharmacodynamics evaluation of metabolic diseases.
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Targeting strategy
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Gene targeting strategy for B-Ldlr KO mice. The exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
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mRNA expression analysis in humanized B-Ldlr KO mice
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Species specific analysis of Ldlr gene expression in wild-type C57BL/6JNifdc mice and homozygous B-Ldlr KO mice by RT-PCR. Liver was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-). Mouse Ldlr mRNA was detectable only in wild-type C57BL/6JNifdc mice, but not in homozygous B-Ldlr KO mice.
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Protein expression analysis
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Western blot analysis of LDLR protein expression in homozygous B-Ldlr KO mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-), and then analyzed by western blot with anti-mouse LDLR antibody (R&D, AF2255). 40 μg total proteins were loaded for western blotting analysis. LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
