B-CAG-luc Daudi Cell Line

Basic Information

Strain Name
Daudi Gt(AAVS1)tm1(luciferase)/Bcgen
Stock Number
B-HCL-020
Common Name
B-CAG-luc Daudi
Disease
Burkitt’s lymphoma
Species
Human
Growth factors
Suspension
Resistence
None
Culture medium
RPMI1640+ 10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
95% Complete medium+5% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-luc Daudi. The luciferase cDNA sequence with CAG promoter was inserted in the AAVS1 locus exon1 of wild-type Daudi .

Details

Phenotypic Analysis

The luminescence signal intensity of B-CAG-luc Daudi cells. The luminescence intensity was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). B-CAG-luc Daudi cells have strong luminescence signal.

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-luc Daudi cells. B-CAG-luc Daudi cells and wild-type Daudi cells (5×106) were subcutaneously implanted into the B-NDG mice (n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-luc Daudi were able to establish tumor in vivo and can be used for efficacy study.

In vivo Imaging Analysis

A.B-CAG-luc Daudi cells were inoculated into the tail vein of B-NDG mice, and imaging was performed on days 0, 3, 7, 10, 14, 17, 21, 24, and 28 for detection and tumor imaging signal intensity analysis.

B.Animal weight monitoring after B-NDG mice were inoculated with B-CAG-luc Daudi cells.

Results: B-CAG-luc Daudi cells were able to establish tumor model steadily and track tumor volume effectively .

 

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