B-CAG-luc U87-MG Cell Line

Basic Information

Strain Name
U87-MG Gt(AAVS1)tm1(luciferase)/Bcgen
Stock Number
B-HCL-015
Common Name
B-CAG-luc U87-MG
Disease
Glioblastoma
Species
Homo sapiens, human
Growth factors
Adherent
Resistence
Puromycin, 0.5μg/mL
Culture medium
MEM+10%FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-luc U87-MG. The hAAVS1 locus of the huamn was inserted into the stop-CAG-Pr-luciferase-PA- LoxP-PuroR- LoxP sequence in B-CAG-luc U87-MG.

Details

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-luc U87-MG cells. B-CAG-luc U87-MG cells (5×106) were subcutaneously implanted into the B-NDG mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-luc U87-MG were able to establish tumor in vivo and can be used for efficacy study.

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