Basic Information
Strain Name
4T1 Gt (ROSA)26Sortm1(luciferase)/ Bcgen
Stock Number
310679
Common Name
B-CAG-luc 4T1
Disease
breast cancer
Species
Mus musculus, mouse
Culture Properties
Adherent
Resistence
0.5ug/ml puro
Culture medium
RPMI1640+10%FBS
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Targeting Strategy
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Details
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Phenotyping Information
Phenotypic analysis of B-luciferase 4T1 cell line
The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase 4T1 cells was significantly higher than that of the control group.
In vivo imaging data analysis of B-luciferase 4T1
B-luciferase 4T1 cells were subcutaneously inoculated with NOD-scid mice and imaged on days 7, 14, and 21.
- The left mouse was inoculated with 5E6 cells, animal number 6; the right mouse was inoculated with 5E5 cells, animal number 5.
- The figure shows tumor volume changes and imaging signal intensity in vivo.
