Phenotypic analysis of B-luciferase 4T1 cell line
The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase 4T1 cells was significantly higher than that of the control group.
In vivo imaging data analysis of B-luciferase 4T1
B-luciferase 4T1 cells were subcutaneously inoculated with NOD-scid mice and imaged on days 7, 14, and 21.
- The left mouse was inoculated with 5E6 cells, animal number 6; the right mouse was inoculated with 5E5 cells, animal number 5.
- The figure shows tumor volume changes and imaging signal intensity in vivo.