Basic Information
Strain Name
HT-29 Tg(luciferase)/Bcgen
Stock Number
B-HCL-011
Common Name
B-luciferase-GFP HT-29 Cell Line
Disease
Colorectal adenocarcinoma
Species
Homo sapiens, human
Growth factors
Adherent
Resistence
Blasticidin S HCl (10 μg/ml, Invitrogen, Cat R210-01)
Culture medium
McCoy’s 5a Medium+10%FBS
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Details
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Phenotyping Information
Phenotypic analysis of B-luciferase-GFP HT-29 cell line
- Imaging under fluorescence microscopy showed green fluorescent expression of B-luciferase-GFP HT-29 cell line
- The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP HT-29 cells was significantly higher than that of the control group.
Imaging data analysis of B-luciferase-GFP HT-29 In vivo
- B-luciferase-GFP HT-29 cells were subcutaneously inoculated with NOD-scid mice and imaged on days 6, 16, and 28. The left mouse was inoculated with 5E6 cells, animal number 4; the right mouse was inoculated with 5E5 cells, animal number 3.
- The image shows the tumor volume change and in vivo imaging signal intensity.
