Homo sapiens, human
Blasticidin S HCl (10 μg/ml, Invitrogen, Cat R210-01)
McCoy’s 5a Medium+10%FBS
90% FBS+10% DMSO
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
Phenotypic analysis of B-luciferase-GFP HT-29 cell line
- Imaging under fluorescence microscopy showed green fluorescent expression of B-luciferase-GFP HT-29 cell line
- The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP HT-29 cells was significantly higher than that of the control group.
Imaging data analysis of B-luciferase-GFP HT-29 In vivo
- B-luciferase-GFP HT-29 cells were subcutaneously inoculated with NOD-scid mice and imaged on days 6, 16, and 28. The left mouse was inoculated with 5E6 cells, animal number 4; the right mouse was inoculated with 5E5 cells, animal number 3.
- The image shows the tumor volume change and in vivo imaging signal intensity.