B-Luciferase-GFP NCI-H1975 Cell Line

Basic Information

Strain Name
NCI­-H1975 Tg (luciferase)/Bcgen
Stock Number
B-HCL-012
Common Name
B-luciferase-GFP NCI­-H1975 Cell Line
Disease
Adenocarcinoma; non­small cell lung cancer
Species
Homo sapiens, human
Growth factors
Adherent
Resistence
Blasticidin S HCl (10 μg/ml, Invitrogen, Cat R210-01)
Culture medium
RPMI­1640+10%FBS
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Details

Phenotyping Information

Phenotypic analysis of B-luciferase-GFP NCI­-H1975 cell line

B-luciferase-GFP NCI-H1975 Cell Line

  1. Imaging under fluorescence microscopy showed green fluorescent expression of B-luciferase-GFP NCI­-H1975 cell line
  2. The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP NCI­-H1975 cells was significantly higher than that of the control group.

Imaging analysis of B-luciferase-GFP NCI­-H1975 In vivo

B-luciferase-GFP NCI-H1975 Cell Line

  1. B-luciferase-GFP NCI­-H1975 cells were monitored for tumor size and animal body weight after subcutaneous inoculation of B-NDG mice.
  2. B-luciferase-GFP NCI­-H1975 cells were subcutaneously inoculated into B-NDG mice on day 19 for imaging detection. The results showed that the B-luciferase-GFP NCI­-H1975 subcutaneous xenograft model can be stably established, and the growth of tumor cells can be effectively tracked by using luciferase.
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