NCI-H1975 Tg (luciferase)/Bcgen
Adenocarcinoma; nonsmall cell lung cancer
Homo sapiens, human
Blasticidin S HCl (10 μg/ml, Invitrogen, Cat R210-01)
90% FBS+10% DMSO
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
Phenotypic analysis of B-luciferase-GFP NCI-H1975 cell line
- Imaging under fluorescence microscopy showed green fluorescent expression of B-luciferase-GFP NCI-H1975 cell line
- The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP NCI-H1975 cells was significantly higher than that of the control group.
Imaging analysis of B-luciferase-GFP NCI-H1975 In vivo
- B-luciferase-GFP NCI-H1975 cells were monitored for tumor size and animal body weight after subcutaneous inoculation of B-NDG mice.
- B-luciferase-GFP NCI-H1975 cells were subcutaneously inoculated into B-NDG mice on day 19 for imaging detection. The results showed that the B-luciferase-GFP NCI-H1975 subcutaneous xenograft model can be stably established, and the growth of tumor cells can be effectively tracked by using luciferase.