B-Luciferase-GFP Raji Cell Line

Basic Information

Strain Name
Raji Tg (luciferase)/Bcgen
Stock Number
B-HCL-010
Common Name
B-luciferase-GFP Raji Cell Line
Disease
Burkitt’s lymphoma
Species
Homo sapiens, human
Growth factors
Suspension
Resistence
Blasticidin S HCl (BSH), 10 μg/mL
Culture medium
RPMI1640+10%FBS+2ug/mL BSH
Frozen
95% (RPMI1640+10%FBS) +5% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Details

Phenotyping Information

B-luciferase-GFP Raji cell line Phenotype analysis

B-luciferase-GFP Raji Cell Line

  1. Imaging under fluorescence microscopy showed green fluorescent expression in B-luciferase-GFP Raji cell line
  2. The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP Raji cells was significantly higher than that of the control group.

Animal imaging in vivo

B-luciferase-GFP Raji Cell Line

  1. B-luciferase-GFP Raji cells were imaged on the 0th, 4th, 6th, 8th, 11th, 15th and 18th day after inoculation of B-NDG mice with 1E6 tail vein.
  2. B-luciferase-GFP Raji cells were inoculated with B-NDG mice and monitored for body weight

 

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