Basic Information
Strain Name
Raji Tg (luciferase)/Bcgen
Stock Number
310670
Common Name
B-luc-GFP Raji
Disease
Burkitt’s lymphoma
Species
Homo sapiens, human
Culture Properties
Suspension
Resistence
Blasticidin S HCl (BSH), 10 μg/mL
Culture medium
RPMI1640+10%FBS+2ug/mL BSH
Frozen
95% (RPMI1640+10%FBS) +5% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Details
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Phenotyping Information
B-luciferase-GFP Raji cell line Phenotype analysis
- Imaging under fluorescence microscopy showed green fluorescent expression in B-luciferase-GFP Raji cell line
- The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP Raji cells was significantly higher than that of the control group.
Animal imaging in vivo
- B-luciferase-GFP Raji cells were imaged on the 0th, 4th, 6th, 8th, 11th, 15th and 18th day after inoculation of B-NDG mice with 1E6 tail vein.
- B-luciferase-GFP Raji cells were inoculated with B-NDG mice and monitored for body weight
