Basic Information
Strain Name
K562 Gt (AAVS1) tm1(luciferase) /Bcgen
Stock Number
310673
Common Name
B-CAG-luc K-562
Disease
Leukemia (CML)
Species
Homo sapiens, human
Culture Properties
Suspension
Resistence
Puromycin, 0.5μg/ml
Culture medium
IMDM+10%FBS
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Targeting strategy
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Details
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Phenotyping Information
Phenotypic analysis of B-luciferase K562 cell line
The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP K562 cells was significantly higher than that of the control group.
In vivo imaging analysis of B-luciferase K562
- B-luciferase K562 cells were inoculated into B-NDG mice (G1 inoculated 5E6 cells, G2 inoculated 1E7 cells) on day 0, 4, 7, 11, 21, 25 days;
- Tumor imaging signal intensity and mouse body weight analysis: B-luciferase K562 cells were inoculated into B-NDG mice (G1 inoculated 5E6 cells, G2 inoculated 1E7 cells) and then subjected to in vivo imaging and body weight monitoring;
The results showed that B-luciferase K562 cells can stably establish a leukemia model and can effectively track the growth rate of tumor cells.
