Phenotypic analysis of B-luciferase K562 cell line
The expression of luciferase was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). The results showed that the fluorescence intensity of B-luciferase-GFP K562 cells was significantly higher than that of the control group.
In vivo imaging analysis of B-luciferase K562
- B-luciferase K562 cells were inoculated into B-NDG mice (G1 inoculated 5E6 cells, G2 inoculated 1E7 cells) on day 0, 4, 7, 11, 21, 25 days;
- Tumor imaging signal intensity and mouse body weight analysis: B-luciferase K562 cells were inoculated into B-NDG mice (G1 inoculated 5E6 cells, G2 inoculated 1E7 cells) and then subjected to in vivo imaging and body weight monitoring;
The results showed that B-luciferase K562 cells can stably establish a leukemia model and can effectively track the growth rate of tumor cells.