We gene edit several different strains of mice, including C57BL/6, Balb/c, DBA2, and NOD-scid. Models can be generated either by EGE™ or ESC-based gene editing technologies. The turnaround time for CRISPR/Cas9 EGE™-based and ESC-based models is 6-8 months and 9-12 months respectively. Mice are often the preferred mammalian model organisms due to their short life cycle and close genetic resemblance to humans. They are also the best studied mammalian model organism with abundant genomic, transcriptomic, proteomic, and phenotypic collections.
- Strain: Laboratory mouse strains are either outbred or inbred . Outbred mice are usually bred to maintain maximum heterozygosity. Inbred mice are usually bred to maintain maximum homozygosity. Inbred strains (also called inbred lines) are individuals of a particular species which are nearly identical to each other in genotype due to long inbreeding. A strain is inbred when it has undergone at least 20 generations of brother x sister or offspring x parent mating, at which point at least 98.6% of the loci in an individual of the strain will be homozygous, and each individual can be treated effectively as clones. C57BL/6(N and J), BALB/c, Fvb, A/J, C3H, CBA, DBA/2 etc. are inbred mouse strains.
- CRISPR/EGE™ or ESC/HR: At Biocytogen, 85% mouse and 100% rat gene editing projects are generated via EGE™ technology. Although EGE™ technology is comparatively cheaper and offers faster turnaround times compared to ESC-based projects, projects where the knockin size is greater than 15kb still necessitate the use of ESC/HR technology.
- Superovulation: Superovulation has been used in the production of transgenic mice since the late 1980s to artificially induce ovulation of large numbers of oocytes from limited numbers of female mice. Superovulation facilitates the generation of genetically engineered mice and reduces the number of animals used.
- Breeding: Breeding is one of the critical steps to generate genetically engineered mouse models. A suitable breeding environment is important to have large litter sizes and shorten the timelines to produce mouse models. Biocytogen’s AAALAC accredited SPF animal facilities provide optimal breeding environments. Our experts design the most efficient breeding scheme to save on costs and time for our customers.
Humanized CD3ε Mouse Model Generation and Validation
mRNA Expression Analysis
RT-PCR analysis of the B-hCD3ε gene: hCD3ε mRNA was detected in splenocytes isolated from homozygous B-hCD3ε mice.
Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD3ε mice were analyzed by flow cytometry.
Results: Mouse CD3+ cells were detectable in the WT C57BL/6 mice, whereas human CD3+ cells were detectable in the homozygous B-hCD3ε mice.
Single Humanized Immune-checkpoint Mice
|B-hCD47 (C) Mice||B-CM-022||BALB/c|
|B-hHAVCR2 (TIM3) Mice||B-CM-032||C57BL/6|
|B-hPD-1 Mice Plus||B-CM-034||C57BL/6|
|B-hPD-L1 (CD274) Mice||B-CM-029||C57BL/6|
|B-hTNFRSF18 (GITR) Mice||B-CM-025||C57BL/6|
|B-hTNFRSF4 (OX40) Mice||B-CM-027||C57BL/6|
|B-hTNFRSF9 (4-1BB) Mice||B-EM-023||C57BL/6|
Double Humanized Immune-checkpoint Mice
Triple Humanized Immune-checkpoint Mice
Cre Mouse Models List
|Product||Product No||Application Fields|
|B-ALB-CreERT2 Mice||B-CM-014||Alb promoter-driven inducible Cre mouse model (liver)|
|B-CAG-tdTomato cKI Mice||B-CM-066||Cre-induced tdTomato expression, for Cre expression profile detection|
|B-CD150-iCreERT2 Mice||B-CM-175||CD150 promoter-driven inducible Cre mouse model (Thymic, T cells and macrophages)|
|B-Col10a1-iCre Mice||B-EM-001||Col10a1 promoter-driven Cre mouse model (mature hypertrophic chondrocyte)|
|B-Col2a1-iCre Mice||B-EM-002||Col2 promoter-driven Cre mouse model (type II collencyte)|
|B-Dmp1-iCre Mice||B-EM-003||Dmp1 promoter-driven Cre mouse model (skeleton)|
|B-Grpr-iCre Mice||B-EM-004||Grpr promoter-driven Cre mouse model (digestive system, nervous system)|
|B-Ins1-iCreERT2 Mice||B-EM-005||Ins1 promoter-driven inducible Cre mouse model (pancreas isletbeta cell)|
|B-Ncr1 (Nkp46)-iCre Mice||B-EM-006||Ncr1 promoter-driven Cre mouse model (NK cell)|
|B-Oxr1-iCreERT2 Mice||B-CM-003||Oxr1 promoter-driven inducible Cre mouse model (frontal lobe adult, cerebellum adult and 24 other tissues)|
|B-Pdgfrb-iCre Mice||B-CM-004||Pdgfrb promoter-driven inducible Cre mouse model (parietal cell of multiple organs, such as skin, intestinal tract, kidney, lung and plexus vasculosus around central nervous system)|
|B-Prrx1-iCre Mice||B-EM-007||Prrx1 promoter-driven Cre mouse model (mesenchyme)|
|B-Sp7 (Osx)-iCre Mice||B-EM-008||Sp7 promoter-driven Cre mouse model (skeleton)|
|B-Syn1-iCre Mice||B-CM-005||Syn1promoter-driven inducible Cre mouse model (brain)|
|B-Tnfrsf11a (RANK)-iCre Mice||B-EM-009||Tnfrsf11a promoter-driven Cre mouse model (lymph gland formation and osteoclast differentiation system)|
|B-Ucp1-iCre Mice||B-EM-010||UCP1 promoter-driven Cre mouse model (mitochondria, brown fat)|
|B-Zeb1-iCreERT2 Mice||B-EM-011||Zeb1 promoter-driven inducible Cre mouse model (tunica media of artery and myocardial cell)|
Other Genetically Modified Mouse Models
|Product||Product No||Application Fields|
|B-Apoe-KO Mice||B-EM-021||Hyperlipidemia, atherosclerosis mice model (Cardiovascular system)|
|B-F8 KO Mice||B-CM-007||Hemophilia mice model|
|B-F9 KO Mice||B-CM-008||Hemophilia mice model|
|B-Il17a-EGFP Mice||B-EM-017||IL17 expression and functional test (gene reporter mice)|
|B-Il17a-Gluc Mice||B-EM-019||IL17 expression and functional test (gene reporter mice)|
|B-db/db Mice||B-CM-012||Diabetes mice model|
|B-ob/ob Mice||B-CM-059||Diabetes mice model|
|B-p53 KO Mice||B-EM-020||Tumorigenesis drug test|
|TGFb1-cKO Mice||B-EM-022||Study on transforming growth factor|