Basic Information

Common name
B-hMSLN MC38
Catalog number
332181
Aliases
MPF, SMRP
  Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-hMSLN MC38 cells have the capability to establish tumors in C57BL/6N mice in vivo and can be used for efficacy studies.
NCBI Gene ID

Description

The human MSLN CDS was inserted into mouse Msln gene, so that the mouse MSLN was not expressed anymore, human MSLN was highly expressed on the surface of B-hMSLN MC38 cells.

Targeting strategy

Gene targeting strategy for B-hMSLN MC38 cells. The exogenous promoter and full coding sequences of human MSLN was inserted to mouse exon 2 in B-hMSLN MC38 cells.

Protein expression analysis

MSLN expression analysis in B-hMSLN MC38 cells by flow cytometry. Single cell suspensions from B-hMSLN MC38 cultures were stained with species-specific anti-MSLN antibodies. Human MSLN were detected on the surface of B-hMSLN MC38 cells, mouse MSLN were not expressed anymore. The 1-A03 clone of B-hMSLN MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hMSLN MC38 cells. B-hMSLN MC38 cells (1×106) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hMSLN MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.