Basic Information

Common name
B-Luc-EGFP HL-60
Catalog number
Acute myelogenous leukemia
Tissue types
Peripheral blood
Peripheral blood
B-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia in vivo.


This B-Luc-EGFP HL-60 cell line expresses firefly luciferase as a marker of HL-60 cells. Fluorescence could be detected in B-Luc-EGFP HL-60 cells.

Targeting Strategy

Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

In vitro Luciferase Activity of B-Luc-EGFP HL-60 cells

Luminescence signal intensity of B-Luc-EGFP HL-60 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc-EGFP HL-60 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP HL-60 cells. The 1-B06 clone of B-Luc-EGFP HL-60 cells can be used in vivo experiments.

Quantitative Imaging Data & Body Weight Changes

B-Luc-EGFP HL-60 cells (1×105) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.

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