Basic Information
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Targeting Strategy
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Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector by lentivirus.
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Phenotypic Analysis
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Luminescence signal intensity of B-Luc-EGFP HL-60 cells. B-Luc-EGFP HL-60 cells displayed strong luminescence signal intensity compared to HL-60 cells, as detected by the Bright-GloTM luciferase Assay system (Promega, Cat E2610).
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In Vivo Imaging Analysis
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Tumor growth and in vivo imaging of B-Luc-EGFP HL-60 cells. B-Luc-EGFP HL-60 cells (1×105) were injected by tail vein into B-NDG mice. (A) Imaging was performed twice a week for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.
